|
Watch video protocols on JoVE - www.jove.com. Journal of Visualized Experiments (JoVE) is a scientific journal publishing video articles on biological experiments filmed at leading research institutions. JoVE is the first video journal indexed in PubMed. To see more of this protocol go to www.jove.com IP-FCM: Immunoprecipitation Detected by Flow Cytometry This video protocol was filmed at the Mayo Clinic. Immunoprecipitation detected by flow cytometry (IP-FCM) is an efficient method for detecting and quantifying protein-protein interactions. The basic principle extends that of sandwich ELISA, wherein the captured primary analyte can be detected together with other molecules physically associated within multiprotein complexes. The procedure involves covalent coupling of polystyrene latex microbeads with immunoprecipitating monoclonal antibodies (mAb) specific for a protein of interest, incubating these beads with cell lysates, probing captured protein complexes with fluorochrome-conjugated probes, and analyzing bead-associated fluorescence by flow cytometry. IP-FCM is extremely sensitive, allows analysis of proteins in their native (non-denatured) state, and is amenable to either semi-quantitative or quantitative analysis. As additional advantages, IP-FCM requires no genetic engineering or specialized equipment, other than a flow cytometer, and it can be readily adapted for high-throughput applications.
|
Video Length: 42
Date Found: March 25, 2011
Date Produced: March 25, 2011
|
|
YouTube |
July 18, 2011
Robot mouth developed by Professor Hideyuki Sawada at Kagawa University in Japan ( Listen to to the original on Wikipedia:
|
YouTube |
July 16, 2011
Now you can find apps and games, rent movies and buy books in Android Market on your phone.  (Books and Movies available only in the U.S.)
|
YouTube |
July 14, 2011
Since a clear winner has emerged for this year’s QQOQQ, I've decided to award it early. The QQOQQ is awarded for a simple question asked in the arrogant expectation that there's no possible answer, and this year there is no competition.
|
YouTube |
July 14, 2011
Don’t forget to subscribe & read the video description for info & other episodes :) ++++++++++++++ Previous Episodes: ++++++++++++++ Justin: Datev: Don't forget to subscribe here for more videos +my twitter: +my google+: +my other channels: Video shot by ...
|
YouTube |
July 13, 2011
Hey guys this is the SIXTH episode in the Tag Your Green Road Trip series I am doing with GE & Howcast. Come back every Wednesday for the premiere of a new episode, and go check out the Tag Your Green Map at . Be sure to add yourself to the map and I could be coming to visit you next! ...
|
|
|
|
|
|
Featured Content
Featuring websites that enhance the internet user’s experience.
|